咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>KU812人外周血嗜堿性白細(xì)胞

人外周血嗜堿性白細(xì)胞

簡要描述:CRL-2099 KU812 人外周血嗜堿性白細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:KU812
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-06-26
  • 訪  問  量:2369

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2099 KU812 人外周血嗜堿性白細(xì)胞

ATCC® Number: CRL-2099™ Price: $329.00

Designations: KU812

Depositors: K Kishi

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens (human)

Morphology: myeloblast

CRL-2099 KU812 人外周血嗜堿性白細(xì)胞

Source: Organ: peripheral blood

Disease: chronic myelogenous leukemia

Cell Type: basophil;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation: Nigata Japan

Isolation date: August 1, 1981

Antigen Expression: CD3; Homo sapiens

alanyl (membrane) aminopeptidase (ANPEP, CD13); Homo sapiens

Cytogenetic Analysis: 58, XY, t(9;22)(q34;q11), i(17q), +1, +4, +6, +6, +8, +der(9)t(9;22), + der(9)t(9;22), +der(11)q(11), +14, +15, +19, +21, +21, -9 [44220]

Age: 38 years adult

Gender: male

Ethnicity: Japanese

Comments: The KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(5) viable cells/ml. . Do not allow the cell concentration to exceed 3 x 10(6) cells/ml.

Medium Renewal: every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 20 to 30 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derivative:ATCC CRL-2100

derivative:ATCC CRL-2101

References: 44220: Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609

44221: Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed: 2460439

44222: Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048

61145: Blom T, et al. Phenotypic characterization of KU812, a cell line identified as an immature human basophilic leukocyte. Eur. J. Immunol. 22: 2025-2032, 1992. PubMed: 1639103


CRL-2099 KU812



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 午夜视频免费国产在线| 欧洲熟妇色xxxx欧美老妇多毛网站| 国产做国产爱免费视频| 中文字幕激情视频| 国产高潮视频在线观看| katsumi精品作品在线播放| 成人无码WWW免费视频| 久久亚洲AV无码精品色午夜麻| 极度另类极品另类| 亚洲国产精品久久丫| 色噜噜在线观看| 国产成人亚洲精品91专区高清| 久久国产精品视频| 狠狠躁日日躁夜夜躁2020| 国产成人一区二区三区视频免费 | 成人网免费观看| 久久午夜福利无码1000合集| 最近最好的中文字幕2019免费 | 久热这里只有精品12| 用舌头去添高潮无码视频| 北条麻妃在线视频| 老妇bbwbbw视频| 国产乡下三级全黄三级| 黄床大片30分钟免费看| 国精品无码一区二区三区在线 | 中国一级毛片视频| 最近中文字幕资源8| 亚洲国产成人综合精品| 欧美日韩国产精品va| 医生女同护士三女| 精品长泽梓在线播放视频| 四虎国产精品永久免费网址 | 精品无人区麻豆乱码1区2区 | 人妻有码中文字幕| 芬兰bbw搡bbbb搡bbbb| 国产午夜福利精品一区二区三区| 色偷偷亚洲女人天堂观看欧| 天天操天天干天天插| 一本一本久久a久久精品综合| 日本高清中文字幕| 久久精品国产999大香线焦|