日韩在线三区_日本在线观看不卡_午夜精品99久久免费_免费成人av电影

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>EA.hy926人臍靜脈細(xì)胞融合細(xì)胞

人臍靜脈細(xì)胞融合細(xì)胞

簡要描述:CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:EA.hy926
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-09-11
  • 訪  問  量:4943

產(chǎn)品分類

Product Category

詳細(xì)介紹


ATCC® Number: CRL-2922™ Price:

Designations:

EA.hy926

Depositors: CS Edgell

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: endothelial

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

Source: Tissue: somatic cell hybrid

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: For-profit institutions must obtain license from University of North Carolina, Chapel Hill.

Antigen Expression: Factor VIII-related antigen; Homo sapiens, expressed

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11,12

D13S317: 11

D16S539: 11,12

D5S818: 11

D7S820: 8,9,10

THO1: 6,8,9.3

TPOX: 8,9

vWA: 14,17

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

Comments: The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.

Atmosphere: air, 95; carbon dioxide (CO2), 5

Temperature: 37.0°C

Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

CRL-2922 EA.hy926

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.

6.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.

7.Incubate cultures at 37C. Subculture when cell concentration reaches between 8 X 10(4) and 1 X 10(5) cells/sq. cm.

Interval: Twice a week

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Preservation: Freeze medium: Complete Growth medium, 95; DMSO, 5

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

CRL-2922 EA.hy926

0.25 (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++):ATCC 30-2101

phosphate-buffered saline:ATCC 30-2200

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402

References: 92902: Edgell CJ, et al. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80: 3734-3737, 1983. PubMed: 6407019

92903: Bauer J, et al. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: contributions of induced gene expression, G-proteins, and integrins. J. Cell. Physiol. 153: 437-449, 1992. PubMed: 1280276

92904: Edgell CJ, et al. Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926. In Vitro Cell. Dev. Biol. 26: 1167-1172, 1990. PubMed: 2079463

CRL-2922 EA.hy926

92905: Rieber AJ, et al. Extent of differentiated gene expression in the human endothelium-derived EA.hy926 cell line. Thromb. Haemostasis 69: 476-480, 1993. PubMed: 8322270






























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日韩在线三区_日本在线观看不卡_午夜精品99久久免费_免费成人av电影
亚洲欧美日韩精品久久久| 欧美一区国产在线| 午夜精品久久久久久| 一区二区三区色| 亚洲精品中文字幕在线| 亚洲高清不卡av| 亚洲国产91| 在线观看欧美黄色| 极品少妇一区二区三区| 国产一区日韩二区欧美三区| 国产日本欧美一区二区三区在线| 国产精品日韩精品| 国产精品无码永久免费888| 国产精品日韩精品欧美在线| 国产精品区免费视频| 国产精品高潮呻吟久久av黑人| 欧美性生交xxxxx久久久| 欧美四级在线观看| 国产精品国产三级国产专播精品人| 欧美亚洲不卡| 国产伦精品免费视频| 国产日韩精品在线播放| 国产一区二区三区四区三区四| 国内免费精品永久在线视频| 国语对白精品一区二区| 黄色工厂这里只有精品| 在线播放国产一区中文字幕剧情欧美 | 亚洲品质自拍| 亚洲欧洲美洲综合色网| 亚洲美女视频网| 亚洲一二三四区| 欧美亚洲综合网| 久久精品欧美日韩| 亚洲乱码国产乱码精品精| 一区二区三区免费网站| 亚洲欧美在线免费| 久久久久久久精| 欧美国产日韩精品| 国产精品扒开腿爽爽爽视频| 国产欧美一区二区三区在线老狼| 国产综合色产在线精品| 亚洲高清视频的网址| 亚洲精品中文字幕有码专区| 亚洲一区二区不卡免费| 欧美一区二区三区在线免费观看| 亚洲国产欧美一区二区三区丁香婷| 日韩视频在线观看国产| 亚洲一区在线观看视频| 久久成人这里只有精品| 欧美大学生性色视频| 国产精品va| 黄色成人小视频| 一区在线视频观看| 韩国av一区二区三区| 免费美女久久99| 欧美精品日韩综合在线| 国产精品大片| 国产自产精品| 亚洲精品一区二区三区不| 一区二区三区久久网| 欧美一区二区免费视频| 亚洲精品自在在线观看| 亚洲欧美日韩另类| 六十路精品视频| 欧美视频久久| 狠狠色狠色综合曰曰| 日韩视频一区二区三区在线播放免费观看 | 午夜一区二区三区不卡视频| 久久综合亚洲社区| 国产精品美女| 亚洲经典自拍| 欧美一区成人| 亚洲午夜精品| 免费观看国产成人| 国产精品中文字幕欧美| 亚洲日本在线观看| 久久国产天堂福利天堂| 亚洲摸下面视频| 欧美激情第10页| 欧美视频中文字幕在线| 欧美偷拍一区二区| 亚洲大片在线观看| 欧美一区二区三区四区在线观看| 夜夜爽99久久国产综合精品女不卡| 久久精品导航| 国产精品v亚洲精品v日韩精品| 亚洲第一区中文99精品| 欧美亚洲三区| 午夜精品久久99蜜桃的功能介绍| 欧美精品播放| 在线看片成人| 久久精品国产综合精品| 小嫩嫩精品导航| 欧美日韩亚洲视频一区| 亚洲国产精品成人综合色在线婷婷 | 国产视频在线观看一区二区三区| 99精品99久久久久久宅男| 亚洲欧洲另类| 久久中文久久字幕| 国产日韩欧美综合精品| 亚洲桃花岛网站| 日韩视频在线免费| 欧美va天堂| 精品成人a区在线观看| 性欧美在线看片a免费观看| 亚洲一二三四久久| 欧美日韩精品高清| 亚洲精品国产精品久久清纯直播| 亚洲国产日韩一区二区| 久久―日本道色综合久久| 国产麻豆精品在线观看| 亚洲一二三四区| 亚洲免费综合| 国产精品日韩专区| 亚洲视频在线观看免费| 亚洲一区二区三区四区五区午夜| 欧美日韩高清一区| 日韩视频免费在线观看| 99在线精品免费视频九九视| 欧美高清在线视频观看不卡| 在线观看中文字幕亚洲| 亚洲国产欧美在线| 欧美电影在线| 亚洲精品欧美极品| 中文在线不卡视频| 欧美日韩一级黄| 99在线精品免费视频九九视| 亚洲天堂av在线免费| 欧美视频中文字幕在线| 亚洲视频 欧洲视频| 午夜天堂精品久久久久| 国产精品一二一区| 午夜久久久久| 久久久久久一区二区| ●精品国产综合乱码久久久久| 亚洲精品国产精品乱码不99| 欧美精品成人| 在线视频亚洲| 久久精品国产77777蜜臀| 激情久久影院| 一本到12不卡视频在线dvd| 欧美午夜精品| 亚洲欧美国产制服动漫| 久久久久**毛片大全| 在线播放中文字幕一区| 日韩一区二区久久| 国产精品久久久久av免费| 午夜亚洲精品| 另类国产ts人妖高潮视频| 亚洲激情欧美| 亚洲欧美日韩国产另类专区| 国产日韩一区二区| 亚洲人精品午夜| 欧美亚日韩国产aⅴ精品中极品| 亚洲女性裸体视频| 免费视频一区| 夜夜躁日日躁狠狠久久88av| 欧美综合国产| 亚洲国产日韩欧美在线动漫| 亚洲图片欧洲图片av| 欧美日韩在线播放三区| 先锋影音网一区二区| 美玉足脚交一区二区三区图片| 亚洲美女色禁图| 欧美在线在线| 亚洲精品免费一二三区| 欧美亚洲日本一区| 在线观看成人网| 亚洲尤物视频网| 精品999在线观看| 亚洲无线一线二线三线区别av| 国产亚洲欧美日韩美女| 日韩视频免费| 国产亚洲欧洲| 一区二区三区免费网站| 国产亚洲欧美另类中文| 9人人澡人人爽人人精品| 国产农村妇女精品一区二区| 91久久精品日日躁夜夜躁欧美| 国产精品国产a级| 亚洲国产视频一区二区| 国产精品家庭影院| 亚洲激情自拍| 国产精品久久久久久超碰| 亚洲国产高清aⅴ视频| 国产精品福利片| 亚洲三级免费观看| 国产午夜精品美女视频明星a级 | 久久国产日韩欧美| 欧美色欧美亚洲另类二区| 久久精品成人| 国产精品久久久久久五月尺| 亚洲国产精品视频一区| 国产精品丝袜91| 9久re热视频在线精品| 好看的亚洲午夜视频在线| 亚洲一区免费在线观看| 亚洲国产精品传媒在线观看| 久久大逼视频| 亚洲一区二区视频|